cellsearch® epithelial cell kit  (CellSearch inc)

Journal: Cancers

Article Title: Molecular Interplay between Dormant Bone Marrow-Resident Cells (BMRCs) and CTCs in Breast Cancer

doi: 10.3390/cancers12061626

Figure Lengend Snippet: Stem-like (CD44+/CD24−) breast cancer CTCs are more likely to be mitotically inactive (Ki67−) than epithelial (PanCK+) CTCs. ( A ) Distribution of Ki67 expression in epithelial and ( B ) stem-like CTCs across 16 patient samples. ( C ) Median fluorescence intensity (MFI) of Ki67 in CTCs. ( D ) Expression of Ki67 in epithelial and stem-like CTCs. Representative dot plots from patient #9 and #10 shown. ( E ) Combinatorial expression of uPAR and int-β1 in epithelial and ( F ) stem-like CTCs. ( G ) MFI of uPAR in CTCs. ( H ) Combinatorial expression of uPAR and int-β1in epithelial and stem-like CTCs. Representative dot plots from patient #9 and #10 are shown. Dot plots from all 16 patients are shown in . CTCs from 16 metastatic breast cancer patients were used for these analyses. P -values were calculated using paired-t tests.

Article Snippet: CTC enumeration of blood from NSG mice was performed using 7.0 mL of blood from healthy donors spiked with 500 μL of mouse blood using the CellSearch Circulating Epithelial Cell Kit (Menarini Silicon Biosystems, Inc.).

Techniques: Expressing, Fluorescence

Journal: Cancers

Article Title: Molecular Interplay between Dormant Bone Marrow-Resident Cells (BMRCs) and CTCs in Breast Cancer

doi: 10.3390/cancers12061626

Figure Lengend Snippet: Validation of ex vivo BMRC/CTC using complementary imaging platforms. ( A ) Detection of ex vivo human CTCs by CellSearch CTC (epithelial) kit. The kit enumerates CTCs using DAPI, Pan-cytokeratin-PE (CK-PE), and CD45-APC staining. Upper panel shows a CTC clustering with a CD45+ cell (lymphocyte); lower panels show an individual CTC. Image resolution achieved in accordance with the USFDA-approved CellSearch platform. ( B ) Immunofluorescent (IF) staining of ex vivo BMRC using GCDFP15/MG cocktail. Image of a BMRC showing red IF staining against mouse primary antibody clones 23A3 (GCDFP-15) and 304-1A5 (MG), and green IF staining for rabbit clone 31A5 (MG). ( C ) IF images of single ex vivo epithelial and stem-like BMRCs and CTCs. ( D ) DEPArray images of ex vivo epithelial and stem-like BMRCs and CTCs. Image magnification: 10×. The DEPArray platform allows the capture, visualization, and downstream interrogation of single CTCs. Since the image resolution and magnification obtained from FDA-cleared CellSearch and DEPArray platforms cannot be altered by the user, we used IF to provide a high-resolution image of captured ex vivo BMRC/CTCs.

Article Snippet: CTC enumeration of blood from NSG mice was performed using 7.0 mL of blood from healthy donors spiked with 500 μL of mouse blood using the CellSearch Circulating Epithelial Cell Kit (Menarini Silicon Biosystems, Inc.).

Techniques: Biomarker Discovery, Ex Vivo, Imaging, Staining, Clone Assay